258 research outputs found
The experimental state of mind in elicitation: illustrations from tonal fieldwork
This paper illustrates how an “experimental state of mind”, i.e. principles of experimental design, can inform hypothesis generation and testing in structured fieldwork elicitation. The application of these principles is demonstrated with case studies in toneme discovery. Pike’s classic toneme discovery procedure is shown to be a special case of the application of experimental design. It is recast in two stages: (1) the inference of the hidden structure of tonemes based on unexplained variability in the pitch contour r emaining, even after other sources of influence on the pitch contour are accounted for, and (2) the confirmation of systematic effects of hypothesized tonal classes on the pitch contour in elicitations structured to control for confounding variables that could obscure the relati on between tonal classes and the pitch contour. Strategies for controlling the confounding variables, such as blocking and randomization, are discussed. The two stages are exemplified using data elicited from the early stages of toneme discovery in Kirikiri, a language of New Guinea. *This paper is in the series How to Study a Tone Language, edited by Steven Bird and Larry HymanNational Foreign Language Resource Cente
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Representing Multiple Dependencies in Prosodic Structures
Association of tones to prosodic trees was introduced in Pierrehumbert and Beckman (1988). This included: (i) tonal association to higher-level prosodic nodes such as intonational phrases, and (ii) multiple association of a tone to a higher-level prosodic node in addition to a tone bearing unit such as a syllable. Since then, these concepts have been broadly assumed in intonational phonology without much comment, even though Pierrehumbert and Beckman (1988)\u27s stipulation that tones associated to higher-level prosodic nodes are peripherally realized does not fit all the empirical data. We show that peripherally-realized tones associated to prosodic nodes can be naturally represented with bottom-up tree transducers. Additionally, multi bottom-up tree transducers provide a way to represent non-peripheral boundary tones and multiple tonal association, as well as multiple dependencies in prosodic structures in general, including prosodically-conditioned segmental allophony
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Tonal marking of absolutive case in Samoan
Samoan is an ergative-marking, (reportedly) non-tonal Polynesian language in which ergative case is marked segmentally, but absolutive case has been said to be unmarked. This paper shows that in fact, a high edge tone co-occurs with absolutive arguments, based on converging evidence from the phonetic and phonological analysis of intonational patterns in the spoken utterances of a systematically varied set of syntactic structures. This empirical observation raises puzzles that probe the nature of the syntax-prosody interface and the relation between tone and intonation: what is the relation between this absolutive high edge tone and: (i) other case markers in Samoan, which are all segmental?, and (ii) other high edge tones in Samoan that co-occur with fronted expressions and coordination? I propose that: (i) the absolutive high edge tone is a tonal case marker that may be related to an apparently moribund stressed, segmental absolutive particle [ˈia], (ii) the high tones that co-occur with absolutives, fronting, and coordination are all syntactically determined and each inserted in the spellout of distinct syntactic configurations, and (iii) there is another class of edge tones which reliably co-occur with pauses—intonational phrase boundary tones—that are not inserted in spellout but by the phonological grammar. While my proposal may seem surprising at first, I show that it fits the current data better than any alternative
Computational Perspectives on Phonological Constituency and Recursion
Whether or not phonology has recursion is often conflated with whether or not phonology has strings or trees as data structures. Taking a computational perspective from formal language theory and focusing on how phonological strings and trees are built, we disentangle these issues. We show that even considering the boundedness of words and utterances in physical realization and the lack of observable examples of potential recursive embedding of phonological constituents beyond a few layers, recursion is a natural consequence of expressing generalization in phonological grammars for strings and trees. While prosodically-conditioned phonological patterns can be represented using grammars for strings, e.g., with bracketed string representations, we show how grammars for trees provide a natural way to express these patterns and provide insight into the kinds of analyses that phonologists have proposed for them.Que la fonologia mostri o no recursivitat sovint va lligat al fet que tingui o no cadenes o arbres en l'estructura de les seves dades. A partir de la perspectiva computacional de la teoria formal del llenguatge i tenint en compte com es construeixen les cadenes i els arbres fonològics, mirem de destriar aquestes qüestions. Mostrem que, fins i tot tenint en compte la limitació de paraules i enunciats en la realització física i la manca d'exemples observables d'incorporació recursiva potencial de constituents fonològics més enllà d'unes poques capes, la recursivitat és una conseqüència natural de l'expressió de generalitzacions fonològiques per a cadenes i arbres. Tot i que els patrons fonològics condicionats prosòdicament es poden representar utilitzant gramàtiques per a cadenes, per exemple amb representacions amb claudàtors, mostrem com les gramàtiques amb arbres proporcionen una manera natural d'expressar aquests patrons i proporcionen coneixement rellevant sobre els tipus d'anàlisis d'aquests patrons que s'han proposat des de la fonologia
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Unbounded Recursion in Two Dimensions, Where Syntax and Prosody Meet
Both syntax and prosody seem to require structures with unbounded branching, something that is not immediately provided by multiple context free grammars or other equivalently expressive formalisms. That extension is easy, and does not disrupt an appealing model of prosody/syntax interaction. Rather than computing prosodic and syntactic structures independently and then selecting optimally corresponding pairs, prosodic structures can be computed directly from the syntax, eliminating alignment issues and the need for bracket-insertion or other ad hoc devices. To illustrate, a simple model of prosodically-defined Irish pronoun displacement is briefly compared to previous proposals
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(In)variability in the Samoan syntax/prosody interface and consequences for syntactic parsing
While it has long been clear that prosody should be part of the grammar influencing the action of the syntactic parser, how to bring prosody into computational models of syntactic parsing has remained unclear. The challenge is that prosodic information in the speech signal is the result of the interaction of a multitude of conditioning factors. From this output, how can we factor out the contribution of syntax to conditioning prosodic events? And if we are able to do that factorization and define a production model from the syntactic grammar to a prosodified utterance, how can we then define a comprehension model based on that production model? In this case study of the Samoan morphosyntax-prosody interface, we show how to factor out the influence of syntax on prosody in empirical work and confirm there is invariable morphosyntactic conditioning of high edge tones. Then, we show how this invariability can be precisely characterized and used by a parsing model that factors the various influences of morphosyntax on tonal events. We expect that models of these kinds can be extended to more comprehensive perspectives on Samoan and to languages where the syntax/prosody coupling is more complex
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The role of time in phonetic spaces: Temporal resolution in Cantonese tone perception
The role of temporal resolution in speech perception (e.g. whether tones are parameterized with fundamental frequency sampled every 10 ms, or just twice in the syllable) is sometimes overlooked, and the temporal resolution relevant for tonal perception is still an open question. The choice of temporal resolution matters because how we understand the recognition, dispersion, and learning of phonetic categories is entirely predicated on what parameters we use to define the phonetic space that they lie in. Here, we present a tonal perception experiment in Cantonese where we used interrupted speech in trisyllabic stimuli to study the effect of temporal resolution on human tonal identification. We also performed acoustic classification of the stimuli with support vector machines. Our results show that just a few samples per syllable are enough for humans and machines to classify Cantonese tones with reasonable accuracy, without much difference in performance from having the full speech signal available. The confusion patterns and machine classification results suggest that loss of detailed information about the temporal alignment and shape of fundamental frequency contours was a major cause of decreasing accuracy as resolution decreased. Moreover, machine classification experiments show that for accurate identification of rising tones in Cantonese, it is crucial to extend the temporal window for sampling to the following syllable, due to peak delay
Investigation of the Effect of Contextual Factors on BIN Production in AAE
Treatments of African American English (AAE) in the literature have focused primarily on morphosyntactic differences from mainstream American English. One these differences is found in the tense and aspect system. While both dialects have the present perfect use for “been”, AAE also has a stressed variant of “been”, termed BIN. This aspectual marker is featured in the literature, but the main focus has been on its prosodic qualities. It differs from present perfect been in that it has the semantics of a remote past marker (Rickford 1973, Rickford 1975, Green 1998). For a comprehensive understanding of AAE’s tense aspect system, both syntactic-semantic and discourse-pragmatic aspects of these markers need to be studied as well. We complete a production experiment with members of an AAE-speaking community in Southwest Louisiana followed by an acceptability judgement task. The purpose of the experiment is twofold. First, it allows us to examine BIN production in canonical BIN environments and non-BIN environments. Second, by paying close attention to the context these environments occur in, we can also examine the influence of discourse-pragmatic factors (LONG-TIME, TEMPORAL JUST, POLAR QUESTIONS) on BIN production in unambiguous environments, as well as in ambiguous environments. The factors LONG-TIME and TEMPORAL JUST are found to be significant predictors of BIN production Furthermore, there is a significant difference in ambiguity, such that the unambiguous contexts predicted BIN slightly less. Overall, the results of the experiment suggest that speakers are consistent in their BIN production for expected BIN environments, but more variable in the non-BIN environments for both unambiguous and ambiguous contexts. This raises the interesting question of why speakers are more variable in the non-BIN environments as well as questioning what the discourse-pragmatic factors are actually capturing. Together, however, it suggests that there are a variety of components that can influence BIN production. Future areas of work could further investigate in regards these components
Individual and Collective Contributions of Chaperoning and Degradation to Protein Homeostasis in E. coli
SummaryThe folding fate of a protein in vivo is determined by the interplay between a protein’s folding energy landscape and the actions of the proteostasis network, including molecular chaperones and degradation enzymes. The mechanisms of individual components of the E. coli proteostasis network have been studied extensively, but much less is known about how they function as a system. We used an integrated experimental and computational approach to quantitatively analyze the folding outcomes (native folding versus aggregation versus degradation) of three test proteins biosynthesized in E. coli under a variety of conditions. Overexpression of the entire proteostasis network benefited all three test proteins, but the effect of upregulating individual chaperones or the major degradation enzyme, Lon, varied for proteins with different biophysical properties. In sum, the impact of the E. coli proteostasis network is a consequence of concerted action by the Hsp70 system (DnaK/DnaJ/GrpE), the Hsp60 system (GroEL/GroES), and Lon
TOP2A and EZH2 Provide Early Detection of an Aggressive Prostate Cancer Subgroup.
Purpose: Current clinical parameters do not stratify indolent from aggressive prostate cancer. Aggressive prostate cancer, defined by the progression from localized disease to metastasis, is responsible for the majority of prostate cancer–associated mortality. Recent gene expression profiling has proven successful in predicting the outcome of prostate cancer patients; however, they have yet to provide targeted therapy approaches that could inhibit a patient\u27s progression to metastatic disease. Experimental Design: We have interrogated a total of seven primary prostate cancer cohorts (n = 1,900), two metastatic castration-resistant prostate cancer datasets (n = 293), and one prospective cohort (n = 1,385) to assess the impact of TOP2A and EZH2 expression on prostate cancer cellular program and patient outcomes. We also performed IHC staining for TOP2A and EZH2 in a cohort of primary prostate cancer patients (n = 89) with known outcome. Finally, we explored the therapeutic potential of a combination therapy targeting both TOP2A and EZH2 using novel prostate cancer–derived murine cell lines. Results: We demonstrate by genome-wide analysis of independent primary and metastatic prostate cancer datasets that concurrent TOP2A and EZH2 mRNA and protein upregulation selected for a subgroup of primary and metastatic patients with more aggressive disease and notable overlap of genes involved in mitotic regulation. Importantly, TOP2A and EZH2 in prostate cancer cells act as key driving oncogenes, a fact highlighted by sensitivity to combination-targeted therapy. Conclusions: Overall, our data support further assessment of TOP2A and EZH2 as biomarkers for early identification of patients with increased metastatic potential that may benefit from adjuvant or neoadjuvant targeted therapy approaches. ©2017 AACR
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